There are so many things that classes don't teach you! This book can instantly teach the new laboratorian things that experienced laboratorians know but won't share such as: whether to autoclave or filter sterilize or how to be approach someone for help. A lab is full of volatile things the most dangerous of which are the other workers! If you've ever made an experienced lab person angry when you've moved their equipment or borrowed their reagents you'll understand how helpful this book is!

I wish I had this information when I was a student.

This is a wonderful resource for any lab that has rotating personnel. Often, experienced researchers don't have the time or interest to orient new people but it's all here, and I would be surprised if even an experienced scientist could read through this whole manual without finding useful information.

The author uses common sense and a casual style, and assumes that the reader knows nothing. Early chapters explore BASIC basics, like getting along with colleagues, getting started the first week, questions to ask, and safety procedures; lab setup and equipment; and organization. Later chapters discuss designing and setting up experiments, using a lab notebook, presenting data, making reagents, including storage and disposal, and working in a sterile environment. Final chapters detail work with eukaryotic cell cultures, bacteria, dna/rna/protein, radioactivity, centrifugation, electrophoresis and a light microscope. Along the way, the reader will learn how to format a journal article, work in a hood, or stay in touch with the PI. The only thing missing is library research, but since this is a bench guide that seems a forgivable omission.

Spiral bound and formatted for easy access and understandability, the volume concludes with a glossary and a good index. Black and white line drawings and pictures identify items found in labs and illustrate techniques from pipetting to making entries in a notebook.

This is not a textbook written - the dream tour you'd like to have each of your technicians or students given if you only had the time! Also handy if a person is having problems and you want to refer them to written material. Any working lab could benefit from the addition of this volume. Fabulous.

lifes of common isotopes, principles of centrifugation to growth media for bacterial strains, to name but a few examples.

The volume follows the new lab worker from his first day on the job through the ins and outs of assimilating into the lab. Tips are provided on who runs the show (the secretary and senior tech) to how to get started (do an experiment the first week). The reader is instructed on how to make friends, when to speak up and when to hold back. While much of this advise is simple common sense, having it made explicit is no doubt reassuring and worthwhile. Dr. Barker makes it clear that the integrity of the data are paramount, as they should be, and this message is explicit. The majority of the volume gets down to the nuts and bolts and getting the experiments done, describing equipment (and providing delightful drawings labelling everything from the shaker to the HPLC), instructing on good technique and providing the kind of useful tables to make sure what needs to get autoclaved does and what doesn't is sterile filtered.

Dr. Barker brings her clear passion for the lab and love of science to this volume, imbuing it with an enthusiasm which is infectious; it serves as a reminder to those of us who have become jaded that as Primo Levi said "The lab is the place for the young and returning there you feel young again with the same sense of excitement and anticipation you have at seventeen". This is a welcome addition to the manuals and guides available. It meets a need for the newcomer and would be worthwhile for the more experienced investigator as well.